Activating Human Adipose Tissue with the β3-Adrenergic Agonist Mirabegron

Kong Y Chen; Robert J Brychta; Nikita S Israni; Alex Jiang; Hannah J Lea; Taylor N Lentz; Anne E Pierce; Aaron M Cypess

doi:10.1007/978-1-0716-2087-8_5

Activating Human Adipose Tissue with the β3-Adrenergic Agonist Mirabegron

Methods Mol Biol. 2022:2448:83-96. doi: 10.1007/978-1-0716-2087-8_5.

Authors

Kong Y Chen^{1

2}, Robert J Brychta^{3

4}, Nikita S Israni^{3

4}, Alex Jiang^{3

4}, Hannah J Lea^{3

4}, Taylor N Lentz^{3

4}, Anne E Pierce^{3

4}, Aaron M Cypess^{5

6}

Affiliations

¹ Diabetes, Endocrinology, and Obesity Branch, Intramural Research Program, National Institute of Diabetes, Bethesda, MD, USA. kong.chen@nih.gov.
² Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA. kong.chen@nih.gov.
³ Diabetes, Endocrinology, and Obesity Branch, Intramural Research Program, National Institute of Diabetes, Bethesda, MD, USA.
⁴ Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
⁵ Diabetes, Endocrinology, and Obesity Branch, Intramural Research Program, National Institute of Diabetes, Bethesda, MD, USA. aaron.cypess@nih.gov.
⁶ Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA. aaron.cypess@nih.gov.

PMID: 35167091
DOI: 10.1007/978-1-0716-2087-8_5

Abstract

An appealing strategy for treatment of metabolic disease in humans is activation of brown adipose tissue (BAT), a thermogenic organ best visualized through ¹⁸F-FDG PET/CT. BAT has been activated to varying degrees by mild cold exposure. However, this approach can cause undesirable stress, and there remains no consensus protocol. Here, we describe standardized methods for both acute and chronic activation of BAT using the orally administered β3-adrenergic receptor (AR) agonist, mirabegron. Acute pharmacological stimulation has enabled quantification of whole-body BAT volume and metabolic activity using PET/CT imaging, and chronic stimulation increases these properties of BAT over time.

Keywords: 18F-FDG PET/CT; Brown adipose tissue; Mirabegron; β3-adrenergic receptor agonist.

MeSH terms

Acetanilides* / pharmacology
Adipose Tissue, Brown
Adrenergic beta-Agonists
Fluorodeoxyglucose F18
Humans
Positron Emission Tomography Computed Tomography*
Thiazoles

Substances

Acetanilides
Adrenergic beta-Agonists
Thiazoles
Fluorodeoxyglucose F18
mirabegron